This manual contains the instructions required to start up and operate the components of the api 4000 system. The api 2000 lcmsms system brings all of the power and performance of. Electron multipliers mass spectrometry, scientific. The api 5000tm lcmsms system is todays most sensitive triple quadrupole mass spectrometer for small molecule analysis. Apci ion source manual pump manual software manual. Typically, the apci probe is used at sample flow rates from 50 to 2000. Use the following information to plan the layout of your lcms. The mfc2000 and ms2000 also have an abbreviated version of the commands that helps cut down on typing time. Fundamentals of lc, ms and lcms 5 comparison of lcms and other techniques 9 applications of lcms 11. The api 3200 lcmsms system is a fullyintegrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all.
When you manually optimize for an analyte, you create an ms acquisition method in tune and calibrate mode. With the success of using gcms as a hyphenated analytical instrument, there was increasing anticipation for integrating lc and ms online, but widespread use of lcms systems did not begin until relatively recently. Streamlined design results in a true benchtop lcmsms system requiring less space in your lab. Mass spectrometers remove target components as ions in a gas phase, then detect the ions under high vacuum. The api 2000 lc ms ms system brings the power and performance of triple quadrupole mass spectrometry to your benchtop. Ab sciex api 2000 lcmsms system manual optimization tutorial 303 kb. Apci ion source manual about this manual 1 about this manual this manual contains the instructions required to operate the atmospheric pressure chemical ionization apci ion source also known as the heated nebulizer source for the q trap and api 2000 lcmsms systems. Lcmsms contract testing laboratory in new delhi, india. Api 3200 lcmsms system reliability, reproducibility, and confidence in your data with an unmatched heritage of technological innovation and dependability, the triple quadrupole api 3200 system offers an attractive blend of performance, throughput, and value. I am also having problems with detecting falcarinol on our ms, both positive and negative mode, with without buffer.
Lc and lc ms quick access to user documentation quick access to user manuals. The increasing role played by liquid chromatography. Autofocus and laser feedback options are also available for automated processes and ergonomic ease of use. Ionizationmass spectrometry analytical sciences december 2000, vol 16, pp 1247. It is a compact, easytouse lcmsms system, brings the power and performance of triple quadrupole ms with a small footprint. This video demonstrates how to acquire data in manual tune mode in analyst software, using ppg tuning as an example. We are equipped with api300 lcmsms which is operating since 2006 and a agilent 6410 since 2010. Highest sensitivity triple quadrupole mass spectrometer for small molecule analysis.
View and download ab sciex api 5000 lc ms ms hardware manual online. Streamlined design results in a true benchtop lc ms ms system requiring less space in your lab. This is due to the development of soft ionization techniques and tandem mass spectrometry, which makes it a sensitive tool for detecting and identifying peptides. This manual will describe the installation, operation, and programming for basic. This is a great starting point for someone that knows little or.
Tunecalibrate lc and ms components monitor variability of metrics over several injections always use the same method for lc and ms, even if it is different than your sample method ask the vendor for performance specifications for both lc and ms parameters note when system performance deviates. The detailed user manual contains detailed diagrams for parts identification and selection. Patented hybrid triple quadrupolelinear ion trap technology takes you far beyond the capabilities of any conventional ion trap, enabling you to screen, identify, and quantitate proteins or small molecules in a single analysis. The figures shown in this manual are for the quaternary version of the degasser. The api 2000 lcmsms system brings the power and performance of triple quadrupole mass spectrometry to your benchtop. The first mass filter allows just the ion of interest to pass through to the collision cell, filtering out any unwanted ions in the process. The api 2000 lcmsms system brings all of the power and performance of triple quadrupole mass spectrometry right to your benchtop. C20007 this standard has been developed from the accumulated knowledge and experience of qualified engineers of the oil, petroleum, petrochemical, chemical, and general bulk liquid storage industry. Acquiring data in manual tune mode in analyst software youtube. The api 2000tm lcmsms system brings all of the power and performance. The applied biosystemsmds sciex api 3200 lcmsms system is a fullyintegrated triple quadrupole mass spectrometry system that delivers outstanding sensitivity and selectivity as well as maximum uptimein an affordable, compact package. Our exclusive contracts with our clients yield a wide range of used mass spectrometers from a number of respected oems, including agilent, thermo scientific, applied biosystems, waters, perkin elmer, and many more. Ms2000 can be configured with precision linear encoders on any axis. Introduction the api 2000 series of lc msms instruments are liquid chromatography lc mass spectrometers ms, which incorporate an.
The popularity of this method is attributed to its superior sensitivity, extraordinary selectivity, and rapid rate of analysis. Introduction the api 3000 series of lcmsms instruments are liquid chromatography lc mass. The api 2000tm lc ms ms system brings the power and performance of triple quadrupole mass spectrometry to your benchtop. Introduction the quality control reference materials qcrm portfolio is a. This video explains the basics of mass spectrometry and why it has become such an important analytical tool.
Although the figures show a 320ms, the information also applies to a 310ms. Introduction the api 2000 series of lc ms ms instruments are liquid chromatography lc mass spectrometers ms, which incorporate an. Our lab has an old lcms with agilent 1100 lc and api 2000 triquad mass spec. Ab sciex api 5000 lcmsms hardware manual pdf download. View and download acr electronics ms 2000m product support manual online. Perkinelmer qsight triple quadrupole lcms ms, including the qsight 100, 200 and 400. About this manual the api 4000 lc ms ms system hardware manual is targeted to operators who are familiar with mass spectrometry but are new to the api 4000 lc ms ms system. Lc and lcms quick access to user documentation quick access to user manuals.
Liquid chromatography mass spectrometry lcms has been an indispensable tool for profiling small differences in expression level of peptidesproteins in complex biological medium 58. The mfc2000 and ms2000 command set mimics ludls command set, so that software written with drivers for ludl stages should be able to run an mfc2000 focus controller and ms2000 stage without modification1. Agilent 1100 hplc equipped with pe sciex api 2000 msms. Liquid chromatographymass spectrometry lcms is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography or hplc with the mass analysis capabilities of mass spectrometry ms. Preparing your lab qsight triple quadrupole lcmsms.
Scientific instrument services provides replacement detectors from the major manufacturers of electron multipliers. Table 1 shows the ions used for mrm and the optimised ms parameters such as collision energy ce and declustering potential dp. Lc msms shares the same high performance pumps and injection systems with lcms but the detector has two sets of mass filters in line, separated by a high energy collision cell. Ab sciex api 2000 qtrap lcms ms system conquer scientific. The site conditions must meet the minimum specifications before the perkinelmer service representative can proceed with. The two main modes of ionisation used in api lcms are electrospray ionisation esi. Api 5000 lc ms ms laboratory equipment pdf manual download. Coupled chromatography ms systems are popular in chemical analysis because the individual capabilities of each technique are enhanced synergistically. Manual optimization is used to optimize compound and ion sourcedependent parameters for the analyte. Abisciex api 2000 lcmsms iet refurbished analytical.
Reproducibility problem on 1100 agilent lc and api 2000 lcmsms january 8 09. The varian representative will unpack the cartons and put the modules on the bench. It delivers exceptional sensitivity, precision, accuracy, linearity, and a wide dynamic range for your multiple reaction monitoring mrm assays. Lcmsms chromatograms of an olive oil sample free from spinosad and fortified with the standard at 20 ngml are showed in fig. Based on the method of sample introduction y ou choose, you can add. The api 2000 lc ms ms system brings all of the power and performance of triple quadrupole mass spectrometry right to your benchtop. Conventions within this manual, the following conventions are used. Equipnet is the worlds leading provider of used mass spectrometers and numerous other types of preowned equipment.
The api 2000 lcmsms system from ab sciex brings the power and accuracy of tandem mass spectrometry to a wide range of applications. The api 3200 lc ms ms system is a fullyintegrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all in an affordable, compact package. High uptime, superior reproducibility, maximal productivity. The applied biosystems api3000 is a triple quadrapole system that offers high sensitivity and performance during operation of all ms modes. The api 2000 system leaves plenty of room for everything else in your lab. Api 3000 instrument 5 api 3000 instrument the api 3000 instruments, besides housing a mass spectrometer, provide connection panels for gas and pump inputs and exhaust, and electronic interfaces with the data acquisition system. Api 3200 lc ms ms system reliability, reproducibility, and confidence in your data with an unmatched heritage of technological innovation and dependability, the triple quadrupole api 3200 system offers an attractive blend of performance, throughput, and value. Figure 1 and figure 2 show suggested layouts for the lcms with the 212lc and different autosamplers. About this manual the api 4000 lcmsms system hardware manual is targeted to operators who are familiar with mass spectrometry but are new to the api 4000 lcmsms system. Lowest limits of detection available for dmpk and admet studies. Used mass spectrometer ab sciex api qtrap 5500 lcmsms. Programming section, common to all ms2000 controller. Other instruments claim to have a benchtop design but use the entire bench. Wash the ms with the syringe pump, fill with the wash solvent appropriate for the method usually the diluent.
The evoq triple quadrupole for liquid chromatography lctq was designed for a singular purposeto reliably quantify thousands of real samples in the fastest sampletoreport time possible. Used in microbiology, molecular biology, proteomics, and drug testing, liquid chromatography mass spectrometers can be used for microorganism detection, protein sequencing. Looking for online definition of lcapi ms or what lcapi ms stands for. Our detectors are used in a wide array of applications including mass spectrometry, rgas, process monitoring, photon detection, and esca. The api 3000 lcmsms system is an enhanced high performance triple quadrupole mass spectrometer with applications in protein and peptide analysis, small molecule analysis in drug metabolism and pharmacokinetic studies. Our lcmsms are being put to use in following areas. The legacy api 3200 system is no longer in production, but may still be available as used or refurbished. Api 2000 instrument the api 2000 instruments, besides housing a mass spectrometer, provide connection panels for gas and pump inputs and exhaust, and electronic interfaces with the data acquisition system. Liquid chromatography mass spectrometry lcms is a hybrid of two analysis techniques that are used to separate, detect, quantify, and identify components of a mixed sample.
Check that the zq functions ok and tune the ms to the best signal. Pesticide residues in foods more than 200 compounds in various matrices. Ab sciex api qtrap 5500 lcmsms mass spectrometer for sale. Running mrm on a api 2000 triquad mass spectrometer. Lowest limits of detection available for dmpk and admet. You can use the procedures to learn how to use the software and the instrument. Using a spikein experiment to evaluate analysis of lcms data.
Use of liquid chromatography with tandem mass spectrometry detection lcmsms for bioanalysis has grown exponentially in the pharmaceutical industry since the 1980s. The what, why and how of mass spectrometry youtube. Lcapi ms is listed in the worlds largest and most authoritative dictionary database of. Created by shula levin protocol lcms preparation of hplc. Lc and lcms quick access to user documentation agilent. The api 5000 lcmsms system is todays most sensitive triple quadrupole mass spectrometer for small molecule analysis.